Elicitation of isoflavonoids in cell cultures of Pueraria tuberosa (L.) (Indian Kudzu)

In present study tissue culture experiments have been done for the enhancement of isoflavonoids via elicitation. The callus and suspension culture have been developed to observe the effect of various concentrations of Cuscuta (as biotic elicitor) and phenyl alanine and NAA (as abiotic elicitor). Cascuta elicitation is found much better elicitor for enhancement of production of studied isoflavonoids viz. Puerarin, Genistin, Daidzein and Genistein. These cultures were incubated on a rotary shaker at 100 rpm (with 2” displacement from the central axis) and 25±0.2 ºC in dark. The cell cultures were subcultured every 4^th week with inoculums density being 125 mg dry weight/100 ml medium (10% v/v). HPLC with UV detector was performed at room temperature 25±0.2 ºC. The culture experiments and HPLC analysis were performed with triplicate and to be found reproducible and precise. Among the tested elicitors the cuscuta (50 mgl⁻¹) has shown highest enhancement effect on total isoflavonoid content thus the biotic elicitors are found to be more effective triggering agent for isoflavonoid induction at late stationary phase cultures.