1Amity Institute of Forensic Sciences, Amity University, Noida, U.P
2Amity Institute of Forensic Sciences, Amity University, Noida, U.P
3Forensic Science Laboratory, G.N.C.T, New Delhi
4Amity Institute of Forensic Sciences, Amity University, Noida, U.P
The present study describes simple, precise, cost effective U.V –Vis Spectroph-otometric method for the estimation of cephalosporin antibiotics in forensic samples. The solvent used throughout the experiment was methanol. The absorption maxima of cephalosporin antibiotics were found to be 249, 241, 253, 238, 239, 239, 241, 247, 241 and 239 nm for Ceftriaxone, Cefixime, Cefepime, Cefpodoxime, Cefazolin, Cefpirome, Cephalexin, Cefotaxime, Cefuroxime and Cefadroxil respectively. Beer law was obeyed in the range of 2-20microgram/ml. The recovery values of cephalosporin antibiotics in forensic samples found to be less than or equal to 70%. Hence the proposed method is applicable for quantitative determination of cephalosporin antibiotics in forensic samples.
Cephalosporin, forensic samples, U.V spectrophotometer
