Journal of Punjab Academy of Forensic Medicine & Toxicology
SCOPUS
  • Year: 2017
  • Volume: 17
  • Issue: 2

Detection and Determination of Pyrimethamine In Body Fluids Using Thin Layer Chromatography

1Research Associate, Amity Institute of Forensic Sciences, Amity University, Uttar Pradesh

2Professor & Director, Amity Institute of Forensic Sciences, Amity University, Uttar Pradesh

* Corresponding Author: Neha Tomar, Research Associate, B- Block, Lower Ground Floor, Amity Institute of Forensic Sciences, Amity University, Sector -125, Noida, Uttar Pradesh- 201313 Email - tomarneh@gmail.com

Abstract

The cases of toxicity induced by drugs have risen tremendously in the past few decades. This pernicious effect is attributed to over dosage and drug reaction. Biological Samples in a case of poisoning was examined for presence of Pyrimethamine using thin layer chromatography. Drug residues were extracted from gastric lavage, vomit and urine employing solvent system extraction method at pH 9. Drug residues obtained were separated and identified by TLC on silica gel G layer with solvent system methanol: ammonia and visualized by potassium iodoplatinate reagent. The method was found applicable with limit of detection up to 0.05 mg/ml

Keywords

Pyrimethamine, Chromatography, GC-MS