Human Genetic Identification in Paternity testing by multiplex STR analysis using ABI 3500 xl Genetic Analyzer

The present study explores the use of 15 STR loci D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA in addition to a gender identification marker, amelogenin in forensic investigation & paternity testing.

The 15 molecular markers were analysed for human identification in a case of disputed paternity. AmpFl STR Identifiler™ plus PCR amplification kit was used for DNA analysis using STRs. The amplified loci were separated through capillary electrophoresis on Applied Biosystems 3500xl Genetic Analyzer as per the manufacturer's recommended protocol. The data was analysed using Gene Mapper™ 1.4 software.

The statistical analysis was performed by Bayesian mathematics using in- house allele frequency data. The paternity index & probability of paternity were 41,431,267.4214075 & 99.9999975863640 respectively. DNA analysis conducted on the samples of mother, child and the disputed father convincingly established the paternity of the child. The Combined Paternity Index (CPI) of the molecular markers & probability of paternity in human identification are very high. The results of analysis of 15 molecular markers alongwith their biochemical characterization (including the chromosomal location of the markers & repeat unit sequence) conclusively establishes the paternity of the child & the genetic identification.