Journal of Punjab Academy of Forensic Medicine & Toxicology
SCOPUS
  • Year: 2019
  • Volume: 19
  • Issue: 2

Identification of the skeletal remains of a murder victim in forensic casework by dna typing: A case report

  • Author:
  • Monika Chakravarty1,1, Prateek Pandya2, Anupuma Raina3, Dhruv Sharma4, Naresh Kumar5, Nidhi Singh6, Damini Varshney7
  • Total Page Count: 3
  • Published Online: Mar 20, 2019
  • Page Number: 168 to 170

1Forensic Science Laboratory Home Department, Govt. of NCT of Delhi, Rohini

2Amity University, Noida

3AIIMS, Delhi

4AD Biology, FSL Delhi

5SSO Biology, FSL Delhi

6LA Biology, FSL Delhi

7SA Biology, FSL Delhi

*Corresponding Author Ms. Monika Chakravarty, Forensic Science Laboratory Home Department, Govt. of NCT of Delhi, Rohini, Contact: +91 98689–31045 e-mail: monika42@gmail.com

Abstract

We report the identification of skeletal remains using DNA technology. DNA was isolated from severely decomposed human remains using pre-extraction decalcification followed by optimized phenol/chloroform DNA extraction procedure. Additionally DNA from the reference samples of the relatives was extracted using the Automate Express DNA extraction system. Quantifiler™ Human DNA quantification kit was used for DNA quantification. AmpFl STR Identifiler plus PCR amplification kit was used to simultaneously amplify 15 autosomal Short Tandem Repeat (STR) loci alongwith the gender specific locus amelogenin.

Keywords

Forensics, Bone, DNA, PCR, STR