Y-27 STR mediated genetic diversity analysis of tibetans of the northern range of Himachal Pradesh, India

Forensic DNA profiling has revolutionized criminal investigations and has been instrumental in solving cold cases, confirming or excluding suspects, and providing crucial evidence in legal proceedings. Additionally, it has been used in humanitarian efforts to identify victims of mass disasters or conflicts, as well as in paternity testing to establish familial relationships. The reliability and discriminatory power of Y STR markers make them essential tools in the field of forensic genetics. Blood samples were collected from 50 healthy male individuals with their written consent. Studying the genetic characteristics of a specific population, such as Tibetans in Himachal Pradesh, helps in understanding the unique genetic traits and variations within that population. The amplification of samples using a Y-chromosomal Short Tandem Repeat (YSTR) marker kit, specifically the one targeting 27 Y-STR markers (Y-Filer Plus™ amplification kit), provides valuable genetic and forensic information. The range of Gene Diversity (GD) values across all 27 loci (from 0.449 to 0.851) suggests a variable level of polymorphism across the Y-STR markers examined. A high gene diversity value suggests a higher level of polymorphism at that particular locus within the studied population. DYS385B marker has turned to be more prominent and polymorphic marker in the analysis of studied population as it showed highest power of discrimination among all markers that is 0.834, highest genetic diversity i.e. 0.851, lowest matching probability i.e. 0.165 and highest value of effective alleles i.e. 6.03 among all other markers.