Journal of Plant Biochemistry and Biotechnology
  • Year: 1992
  • Volume: 1
  • Issue: 2

Cloning and Characterization of a Foldback DNA Element from Antirrhinum majus

  • Author:
  • Kailash C Upadhyaya
  • Total Page Count: 3
  • Page Number: 93 to 95

Abstract

A foldback sequence linked to a copy of the transposable element, Tam2, has been isolated, from a genomlc library constructed using partially digested and size-fractionated Antirrhinum DNA into λ EMBL4 vector, by using Tam2 specific probe. The foldback sequence visualized by heteroduplex analysis reveals a typical stem-loop structure. The foldback element is around 1.2 kb with an average of 227 bp of inverted termini forming the stem.

Keywords

foldback sequence, Antirrhinum majus, heteroduplex analysis