Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
*Corresponding author, Biotechnology Centre, Punjab Agricultural University, Ludhiana-141004, Punjab, India.
**Central Institute of Medicinal and Aromatic Plants, Post Bag No. 1, P.O.-RSM Nagar, Lucknow-226016, Uttar Pradesh, India.
Plasmid DNA (CaMVGUS, containing β-glucuronidase gene) was used to transform developing seed of wheat and tobacco through pollen-tube pathway. The DNA was applied on the ovule end of excised styles and stigmas of wheat and tobacco after pollination. None of the 272 wheat and 13,567 tobacco plants obtained after application of the isolated DNA showed β-glucuronidase activity suggesting that the pollen-tube pathway may not be effective for transformation of plants.
transformation, pollen-tube, wheat, tobacco, β-glucuronidase
