aDepartment of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110 021, India
bDepartment of Biotechnology, Chaudhary Charan Singh University, Meerut 250 004, India
*Corresponding author. Fax: 91-011-4675095; E.mail: aktpmb@hotmail.com
†This sequence of the cDNA reported is deposited in EMBL database with the accession No. AJ296743.
An expression assay of randomly selected cDNAs resulted in the isolation of a novel cDNA (OSISAM) of SAM synthetase from rice. Nucleotide sequence data show that the cDNA is similar to a genomic clone (pRSAM1) from rice in the coding region but differs in the 5′ untranslated region (UTR) that is unique to this cDNA. The RNase protection experiments and search of EST database confirmed the presence of such novel cDNA. Northern analysis shows that the gene is differentially expressed in various tissues. The transcript abundance also increases when plants are stressed with salt, drought, ABA, injury, osmotic- or heat-shock, but it is not influenced by cold stress.
S-adenosyl-L-methionine synthetase, cDNA, gene expression, rice, leader sequence, stress-inducible
