In vitro Clonal Multiplication of Acorus calamusL

Acorus calamus L(sweet flag) is a semi-aquatic perennial medicinal herb having a creeping and much branched aromatic rhizome. Rhizome buds cultured in MS basal medium supplemented with BAP (8.87μM) and NAA (5.37μM) at light intensity of 30μE m⁻²s⁻¹ and photoperiod of 12 h produced 8–10 multiple shoots by the eighth week of inoculation. Shoots could be rooted in MS basal medium without growth regulators. They were transplanted in sand and kept in shade for 4 to 5 weeks. 90–95% of the plants were established and were transferred to field after one month.