Thidiazuron Induced Regeneration in Cuminum cyminum L

In this communication we report shoot organogenesis from hypocotyl explants in cumin (Cuminum cyminum) genotype RZ-19 by the use of thidiazuron (TDZ). Various levels of TDZ were incorporated in MS basal medium to induce regeneration. Regeneration was achieved with a frequency up to 30% on 0.5 and 0.1 mg I⁻¹ concentration of TDZ. Shoots once produced could be multiplied on 0.5 mg I⁻¹ kinetin (KN) at the rate of approximately 8 shoots per regenerated shoot. These multiplied shoots could go through 3–4 multiplication cycles after which they root on 1.0 mg I⁻¹ IAA.