Centre for Biochemical Technology, Delhi University Campus, Mall Road, New Delhi, 110 007, India
*Corresponding author. E-mail: rhdas2001@yahoo.co.in
AbbreviationsIAA
indole-3-acetic acid
IBAindole-3-butyric acid
BAP6-benzyl aminopurine
Natural peanut agglutinin (PNA) gene is expressed with a signal sequence of 23 amino acids and a C terminal peptide of 14 amino acids. Functionally active recombinant PNA having apparent subunit molecular weight of 29kD was obtained when expressed without signal peptide and non-essential C terminal peptide sequences in insect cells. Expression in insect cells (Sf9) was driven by a 129bp Spodoptera litura nucleopolyhedrosis virus (SNPV) sequence containing its polyhedrin promoter.
peanut agglutinin gene, recombinant lectin, polyhedrin, S/NPV, AcNPV
