In vitro Propagation and Conservation of Atropa acuminata Royle ex Lindl -An Indigenous Threatened Medicinal Plant

A micropropagation method has been developed for multiplication and conservation of Atropa acominata by induction of axillary shoot proliferation from shoot tips and nodal explants using Murashige and Skoog (MS) medium supplemented with BAP (1 mg I⁻¹) and IBA (1 mg I⁻¹). Revised tobacco (RT) medium with IAA (1 mg I⁻¹) was found most suitable for shoot elongation. Rooting was highest on full strength RT medium containing IBA (1 mg I⁻¹). In vitro raised plantlets were hardened and transferred to soil.