1Department of Plant Agriculture, Biotechnology Division, Crop Science Building, University of Guelph, Guelph, Ontario, Canada, NIG 2WI
2Department of Biology, University of Waterloo, Ontario, Canada, N2L 3G1
*Corresponding author: E-mail: glick@sciborg.uwaterloo.ca
Abbreviations: α-AB, α-aminobutyric acid; ACC, 1-aminocyclopropane-1-carboxylic acid; LRU, light-responsive unit; PR, pathogensis related; ROS, reactive oxygen species.
Transgenic tomato plants with 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase gene from Enterobacter cloacae UW4 under the control of a pathogenesis-related promoter (prb-1b) from tobacco were challenged by abiotic stresses to determine the expression patterns of the transgene. No ACC deaminase RNA or protein was detected by RT-PCR and in western blots prepared from leaf proteins of transgenic plants after wounding or treatment with cc-amino butyric acid, xylanase, ethephon, salicylic acid, jasmonic acid, ethylene, or ethylene plus jasmonic acid. However, expression of the ACC deaminase transgene was observed in leaves and roots of transformed tomato lines exposed to UV light. The UV response required a minimum of 48 h of exposure and was specific to UV-B light.
ACC deaminase, ACGT-containing element (G-Box), ethylene, GCC-element (GCC-Motifs), Prb-1b promoter, UV-B light
