Plant Tissue Culture Laboratory, Department of Botany, University of Jammu, Jammu 180 006, India
*Corresponding author
Abbreviations: BAP, 6-benzylaminopurine; IAA, indole-3-acetic acid; IBA, indole-3-butyric acid; Kn, Kinetin; NAA, α-naphtha-lene acetic acid; MS, Murashige and Skoog.
Callus was initiated from petiole explants of Heracleum candicans on MS medium fortified with BAP and 2,4-D (0.5 mg l−1 each). Maximum shoot differentiation from callus occurred on MS medium containing 1 mg l−1 BAP and 0.2 mg l−1 NAA. The regenerated shoots were rooted on MS medium supplemented with 1 mg l−1 IBA. The rooted plants were transferred to the field after successful hardening in pots containing vermiculite. All regenerated plants were diploid with 2n=22 chromosomes in their root tip cells.
Heracleum candicans, organogenesis
