1National Food Research Institute, Kannondai 2-1-2, Tsukuba, Ibaraki, Japan 305
2Chemistry Department, Indian Institute of Technology, Delhi 110016, India
3National Research Centre for Agricultural Utilization Research, Peoria, Illinois 61604, USA
*Corresponding author. E-mail: khare_sk@hotmail.com
Chromobacterium viscosum lipase was modified by stearic acid in two-phase system for improved trans-esterification (TE) activity. The stearic acid, dissolved in n-hexane, was mixed with lipase in buffer phase for 24 h. The modified lipase was present as insoluble layer at the interface, showed 9 units trans-esterification activity. The FTIR spectrum of lipase showed a new sharp peak corresponding to 1700 cm−1 as a result of stearic acid modification. The modified lipase catalyzed trans-esterification is useful in preparing structured lipid, with capric rich oleic triglyceride as a model system.
lipase, Chromobacterium viscosum, modification, trans-esterification, stearic acid
