aDepartment of Horticulture, Faculty of Agriculture, University of Cukurova, 01330, Balcali, Adana, Turkey
bDepartment of Horticulture, Faculty of Agriculture, University of Mustafa Kemal, 31034, Antakya, Turkey
cFaculty of Engineering and Natural Sciences, University of Sabanci, 81474 Tuzla, Istanbul, Turkey
dDepartment of Virology, ARO Volcani Center, FOB 6, Bet Dagan 50250, Israel
*Corresponding author. E-mail: yesimcan@mail.cu.edu.tr
The optimization of regeneration protocol for different genotypes increases the yield in transformation studies. Cotyledon explants of benzyl adenine (BA) (0, 5, 10, 20 μM) and indole-3-acetic acid (IAA) (0, 0.5, 5 µM). Maximum shoot growth and subsequent rooting from explants on regeneration medium were obtained from the media containing 10 µM BA + 0.5 µM IAA and 20 µM BA (75 and 78%) by direct organogenesis, respectively. Histological analysis showed that cell division was observed in the epidermal and subepidermal layers. Protuberant structures were observed in tissues between 7 and 12 days in culture. Meristematic structures were observed after 12 days in culture which later developed into buds.
growth regulator, histology, regeneration, watermelon
