Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110 012, India
*Corresponding author. E-mail: shellypraveen@hotmail.com
Abbreviations: BAP, 6-Benzyl amino purine; NAA, α-naphthalene acetic acid; MS-Murashige and Skoog.
Somatic embryogenesis and plant regeneration from stem explants of kinnow mandarin, a hybrid of king and willow mandarins, are reported. It is an economically important cash crop of India with great deal of production and export. This is for the first time that callus induction and internodal regeneration has been successfully achieved in this citrus cultivar. Callus was induced from nodal segment (1–2 cm) of kinnow mandarin on Murashige and Skoog (MS) medium supplemented with a-naphthaleneacetic acid (NAA) and benzyl amino purine (BAP). Cotyledonary stage somatic embryos with regenerated shoots were induced on BAP-enriched medium. Roots developed when regenerated shoots were excised and cultured on half strength MS medium supplemented with NAA only.
callus, internodal regeneration, Citrus sinensis
