Department of Plant Breeding, Genetics & Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India
*Corresponding author. E-mail: hsdhaliwal76@hotmail.com
Abbreviations: NIL, near isogenic line; SCAR, sequence characterized amplified region; SSR, simple sequence repeats; STMS, sequence tagged microsatellite.
Leaf rust resistance gene Lr28 has been transferred form Aegilops speltoides into bread wheat on chromosome 4AL. To identify the molecular markers linked to Lr28 the available microsatellite markers for wheat chromosome arm 4AL were surveyed on near isogenic lines (NILs) of Triticum aestivum cultivars having Lr28 gene, other Lr genes and susceptible cultivars. A null allele of Xgwm 160 marker was found to be associated with Lr28. Linkage between the marker and the Lr28 resistance gene was confirmed using F2 mapping population of cross PBW343 and HD2329 + Lr28.
Triticum aestivum, STMS, SCAR, leaf rust resistance genes, Aegilops speltoides
