In vitro regeneration of gerbera jamesonii bolus from leaf and petiole explants

Regeneration of adventitious shoots from leaf and petiole pieces of Gerbera jamesonii has been obtained on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins and cytokinins. About 75–77 per cent of the calli from both types of the explants produced 12–15 shoots per callus with 3 mgl⁻¹ BAP. Auxins and kinetin, separately failed to produce shoots. The shoots regenerated on the callus induction medium (CIM). The regenerated shoots multiplied with 1 mgl⁻¹ BAP, were rooted on MS medium containing 1 mgl⁻¹ BAP + 0.1 mgl⁻¹ IAA. The plants obtained were transferred to pots and acclimatized with 60–70 per cent success.