1Department of Botany, D.D.U. Gorakhpur University, Gorakhpur 273 009, India
2Department of Botany, Saint Andrew's Post Graduate College, Gorakhpur 273 001, India
*Corresponding author. E-mail: neerajsrivas@yahoo.com
Abbreviations: BA Benzyladenine; 2,4-D, 2,4-dichloro-phenoxyacetic acid; Kn, Kinetin; NAA, Napthalene acetic acid; IAA, Indole acetic acid; IBA, Indole butyric acid.
Paedaria foetida L, which has great medicinal value is facing danger of extincion. For its conservation, in vitro multiplication may prove one of the best techniques. Micropropagation of P. foetida has been achieved through the culture of nodal explants. The explants produced shoots on MS medium with 0.8% agar. This medium, however, caused high degree of browning of the explants and death of sprouted shoots. Agar free liquid MS medium with polyvinylpyrrolidone (PVP) proved better. Maximum shoot proliferation, free from callus and vitrification but with poor rooting could be obtained in liquid MS medium with PVP (0.8%), NAA (0.5 mg l−1) and BA (2.0 mg l−1). The best rooting occurred on semisolid MS medium containing 0.8% agar and 0.5 mg l−1 IBA.
micropropagation, Paedaria foetida, medicinal plant
