1Department of Botany, SP College, Srinagar 190 001, India
Plant Tissue Culture Laboratory, Department of Botany, Jammu University, Jammu 180 006, India
*Corresponding author
Abbreviations: BAP, 6-benzylaminopurine; IAA, indole-3-acetic acid; IBA, indole-3-butyric acid ; Kn, Kinetin; NAA, α-naphthalene acetic acid ; MS, Murashige and Skoog.
A protocol is described for rapid and large-scale in vitro propagation of Anethum graveolens by enhanced axillary shoot induction that was dependent on BAP supply. The synergistic combination of 0.5 mg l−1 BAP and 0.1 mg l−1 IBA induced 100% shoot formation as well as shoot number (6.6 ± 0.48 per explant). Subculturing of shoot tips of in vitro plants on multiplication medium enabled continuous production of healthy shoots with similar frequency. Rooting of shoots was achieved on a medium with 1mg l−1 IBA and 0.5 mg l−1 Kn. Micropropagated plants established in garden were uniform and identical to the donor plant with respect to morphological and cytological characteristics.
axillary shoot proliferation, essential oil, herbal medicine
