1Plant Molecular Biology Laboratory, Rajiv Gandhi Centre for Biotechnology, Jagathy, Thycaud P O, Thiruvananthapuram 695 014, Kerala, India
2Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 585 MCLM Building, 1918 University Boulevard, Birmingham, Alabama, AL 35294, USA
3Neurobiology Laboratory, Rajiv Gandhi Centre for Biotechnology, Jagathy, Thycaud P O, Thiruvananthapuram 695 014, Kerala, India
*Corresponding author: E-mail: purshee@rgcb.res.in
Hydroxymethylglutaryl-CoA (HMGCoA) reductase, a key enzyme of the mevalonate pathway is known to play a key role in the biosynthesis of terpenoids. Here we describe, the cloning and characterization of hmgr1 promoter from Andrographis paniculata. A 710 bp partial promoter fragment including a putative TATA box, 5′ UTR and 5′ coding sequence of the gene was cloned by amplifying the upstream sequences using PCR with appropriate primers. Analysis of the promoter sequence against PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative light dependent regulatory cis-elements in the sequence along with TATA and CAAT boxes. RT-PCR detected GUS transcripts in transgenic plants.
Andrographis paniculata, HMGCoA reductase, promoter, genome walking
