Department of Botany, University of Delhi, Delhi 110 007, India
*Corresponding author: E-mail: babbars@rediffmail.com
Abbreviations: AdS: Adenine Sulphate; kn: Kinetin; BAP: 6-benzylaminopurine; NAA: α-naphthalene acetic acid; 2,4-D: 2, 4-dichlorophenoxy acetic acid; IAA: Indole-3-acetic acid; IBA: lndole-3-butyricacid; CH: Casein hydrolysate; MS: Murashige and Skoog's medium.
A protocol for regular in vitro regeneration of Carthamus tinctorius var HUS-305 is described. The morphogenic response of seed explants and explants from seedlings of different ages were studied on Murashige and Skoog's medium (MS) supplemented with different growth regulators. The protocol finally standardized involves culture of cotyledonary explants from 2 cm long, 2-to 6-day-old seedlings on MS supplemented with 1.0 mg l−1 BAP and 0.1 mg l−1 kinetin. Various other adjuvants viz., adenine sulphate, glutamine and casein hydrolysate were also tested. None of these promoted the caulogenic response significantly. The microshoots could be rooted on medium supplemented with different auxins. The highest rhizogenic response was on ½ MS supplemented with 0.2 mg l−1 NAA.
caulogenesis, growth regulators, cotyledonary explants, safflower
