Biotechnology Laboratory, Department of Botany, University of Rajasthan, Jaipur 302 004, India
*Corresponding author Present address: 27-A, Vasudevpuri, Kalwad Road, Jhotwara, Jaipur 302 012
Abbreviations: BAP, 6-benzylamino purine; IBA, Indole-3-butyric acid; MS, Murashige & Skoog's; SE, Standard error.
An efficient regeneration method via shoot tip explant has been developed for Jatropha curcas, which is a medicinally as well as economically important plant. Shoot tips were proliferated on MS medium incorporated with BAP (2.0 mg l−1) and IAA (0.5 mg l−1) along with adenine sulphate, glutamine and activated charcoal. In vitro produced shoots were induced to root on IBA (0.5–5.0 mg l−1) added to half strength MS medium. The highest frequency of root induction was on the medium with 3.0 mg l−1 IBA. Regenerated plantlets were successfully transferred to field after initial acclimatization.
Jatropha curcas, shoot tip, regeneration
