1Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200 030, People’s Republic of China
2State Key Laboratory of Genetics Engineering, Institute of Genetics, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai 200 433, People’s Republic of China
**Corresponding author. E-mail: kxtang1@yahoo.com or kxtang1@163.com
*Kaijing Zuo and Jingya Zhao contributed equally to this paper.
The full-length enolase-encoding cDNA was cloned from cotton (Gossypium barbadense). This gene (GenBank Accession No.: AY297757) had a total length of 1580 bp with an open reading frame of 1338 bp and encoded a predicted polypeptide of 445 amino acid residues with a molecular weight of 47.73 kD. Comparison of it primary structure with those of other enolases revealed a remarkable degree of conservation, except for the presence of an insertion of 5 amino acid residues unique to higher plant enolases. Southern blotting analysis of genomic DNA indicated enolase was likely to be a low-copy gene in the cotton genome. The enolase gene was induced in response to submergence, ABA, salt and high temperature stress. Our studies suggested that the cloned gene was a new member of plant enolase gene family, which contributed to the energy supply in stress-treated tissues.
cDNA cloning, enolase, cotton, Gossypium barbadense
