1Division of Seed Science & Technology, Indian Agricultural Research Institute, New Delhi 110 012
2School of Biotechnology, Guru Gobind Singh Indraprastha University, Delhi 110 006
3School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067
*Corresponding author. E-mail: vibha_k_75@yahoo.co.uk
Present address: D II/12, Kaka Nagar, New Delhi 110 003
Molecular characterization of 32 Indian rice varieties of different agro-climatic zones resulted in mean heterozygosity value of 0.622, 0.819 and 0.890 over polymorphic loci and marker index value of 1.00, 6.75 and 4.16, respectively for RAPD, ISSR and STMS primers. The three marker systems resulted in 201 polymorphic bands (94.36%) out of a total of 213 bands. The probability of a chance identical match between two varieties was very low (2.08x10−10) in combined molecular marker analysis as compared to individual marker system (RAPD, 7.5 x 10−4 ; ISSR, 1.5 x 10−3 and STMS, 3.9 x 10−6). The combined average genetic similarity for molecular markers and coefficient of parentage (COP) across all 496 pairwise combinations revealed a non-significant relationship (r = 0.215).
genetic similarities, molecular markers, PCR, Oryza sativa, rice varieties
