Department of Botany, University of Rajasthan, Jaipur 302 004, India
*Corresponding author. E-mail: slkothari@lycos.com
Abbreviations: ABA-Abscisic acid; BA-Benzyladenine; 2,4-D-2,4-dichlorophenoxyacetic acid; GA3-Gibberellic acid; IBA-Indole-3-butyric acid; MS-Murashige and Skoog; TBA-Tertiary butyl alcohol.
An efficient protocol for direct somatic embryogenesis in Capparis decidua has been developed. Mature zygotic embryos cultured in Murashige and Skoog (MS) liquid medium with 2,4-D (0.1 mg l−1) and BA (0.5 mg l−1) produced somatic embryos directly without an intervening callus phase from the subepidermal cells. Treatment with ABA promoted maturation of somatic embryos and BA (1 mg l−1) promoted germination. One zygotic embryo produced approximately 230 somatic embryos within 14 to 15 weeks. Embryos germinated in eight weeks and acclimatized plants were transferred to pots.
Capparis decidua, somatic embryogenesis, zygotic embryos
