Molecular and histochemical analysis of a t-dna tagged mutant of Arabidopsis exhibiting anther - specific gus expression

An Arabidopsis thaliana mutant, exhibiting anther specific GUS expression, identified from a mutant population of Arabidopsis tagged with a promoterless β-glucuronidase (GUS), carries the T-DNA insertions at two distinct loci. We have been able to segregate the two inserts from each other by backcrossing with wild type plants. The insertion responsible for anther specific GUS expression in segregating population has been identified and confirmed to be in the upstream region of a putative peroxidase gene, AT2G24800. Here we report detailed histochemical and molecular characterization of the mutant Anth85, carrying a single insertion of T-DNA in the peroxidase gene. In Anth85, the GUS expression was observed in the anthers and rosette of the young seedlings. The expression of GUS in the anthers was restricted to the tapetum and microspores. The mutant has no developmental defects and the gene appears to be redundant for normal plant growth. Cloning of upstream region and detailed deletion study of upstream region in transgenic plants is likely to lead to the identification of anther specific promoter elements.