Subspecies – Specific microsatellite markers for rice (Oryza sativa L)

Subspecific classification of Asian rice (Oryza sativa L) into indica and japonica has always been a subject of interest althrough for rice breeders and geneticists. The present study aims at identifying subspecies specific microsatellite markers in six genotypes, each of indica and japonica using 372 microsatellite primers covering the entire genome. Only 36 primers gave clear polymorphism on 3% agarose gel and these can be used as diagnostic markers for routine and easy identification of the subspecies.