Simple and effective regeneration of mungbean (Vigna radiata (L) Wilczek) using cotyledonary node explants

Mungbean (Vigna radiata (L) Wilczek cv ML – 267) is a recalcitrant grain legume species. Direct multiple shoots were developed from the cotyledonary node explants of 2-day-old in vitro grown seedlings of mungbean. Maximum number of shoots (an average 12.1 shoots per explant) was obtained on a medium containing MS salts, B₅ vitamins and 5.0 mg l⁻¹ BAP. A medium with lower BAP concentration appeared suitable for rapid shoot elongation. The elongated shoots were rooted on 0.2 mg l⁻¹ NAA. The rooted plants were acclimatized under field conditions. The survival of the plants in the greenhouse was 90%. Plants flowered and set seed normally.