Journal of Plant Biochemistry and Biotechnology
  • Year: 2007
  • Volume: 16
  • Issue: 1

Proline accumulation in transgenic tobacco as a result of expression of Arabidopsis Δ1-Pyrroline-5-carboxylate synthetase (P5CS) during osmotic stress

  • Author:
  • A Yamchi1,2, F Rastgar Jazii1,, A Mousavi1, A A Karkhane1, Renu 1,3
  • Total Page Count: 7
  • Page Number: 9 to 15

1National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. BOX 14155 6343, Tehran, Iran.

2Department of Plant Biotechnology, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran.

3Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110 012, India.

*Corresponding author: E-mail: rastgar@nrcgeb.ac.ir, rastgar_jazii@yahoo.com

Abstract

The entire coding sequence of the bi-functional enzyme, Δ1-Pyrroline-5-carboxylate synthetase (P5CS) from Arabidopsis thaliana was reverse-transcribed, amplified and expressed under the control of CaMV 35S promoter in transgenic tobacco plants. Several lines were established and tested for the expression of P5CS. Drought and salinity were applied as osmotic stresses and proline content of the transformed plants was compared with that of non-transformed controls. Results indicate that transgenic lines express higher levels of proline and show enhanced resistance to the applied osmotic stress as compared to the non-transgenic plants.

Keywords

cDNA cloning, Δ1-Pyrroline-5-carboxylate synthetase, Arabidopsis, osmotic stress, drought tolerance, proline, tobacco transformation