Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, India.
*Corresponding author: E-mail: neelam@nbpgr.ernet.in.
AbbreviationsBA
6-benzyladenine;
IAAindole-3-acetic acid;
IBAindole-3-butyric acid;
MSMurashige and Skoog's medium;
NAAα-naphthaleneacetic acid;
spermidineN-(3-aminopropyl) butane-1,4-diamine.
Shoot multiplication of Curculigo orchioides Gaertn (Kali Musali), a threatened medicinal herb, was achieved using shoot bud explants. Single explants cultured on MS + 0.2 mg l−1 BA + 2.5 mg l−1 spermidine produced multiple shoots with an average of 7 shoots per explant that readily rooted on the same medium. Plantlets were acclimatized before successful transfer to soil. The developed protocol demonstrates the use of spermidine for mass propagation that can help in conservation of germplasm of Curculigo orchioides.
Curculigo, in vitro conservation, in vitro propagation, Kali musali, spermidine, threatened plant
