1National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, India
*Corresponding author. E-mail: jlkarihaloo@rediffmail.com
2Present address: Asia-Pacific Consortium on Agricultural Biotechnology, CG Centres Complex, Dev Prakash Shastri Marg, New Delhi110 012, India
Twelve Indian mango cultivars were fingerprinted using anchored-ISSR primers. Out of total 161 bands amplified, 113 (70.2%) were polymorphic, the polymorphism ranging from 50% to 94.1% depending upon the primer. One primer [5′ HVH(CA)7T 3′] with highest genotype index could uniquely identify each of the cultivars studied. Fingerprints based on polymorphic markers amplified by the 10 primers had a 6.75 x 10−12 probability of identical match by chance, indicating a high degree of uniqueness in the anchored-ISSR based fingerprinting. UPGMA dendrogram based on Jaccard's similarity showed Himayath as the most diverse of the 12 cultivars and the rest of the cultivars clustered into two groups at 0.69 similarity coefficient.
Mangifera indica, DNA fingerprinting, Anchored-ISSR markers
