1Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 447 Sri-ayudthaya road Bangkok 10400, Thailand
2Department of Plant Science, Faculty of Science, Mahidol University, Rama 6 road, Bangkok 10400, Thailand
*Corresponding author. E-mail: pyspr@mahidol.ac.th
AbbreviationsBA
6-Benzylaminopurine,
MS mediumMurashige and Skoog medium,
PGRPlant growth regulator,
TDZThidiazuron.
A micropropagation protocol for Gagnepainia godefroyi K Schum and G thoreliana (Baill) K Schum, two rare medicinal plants of Thailand, has been developed using terminal buds. After a total of twenty weeks of culture (4 weeks on MS added with 36.33 μM TDZ and 16 weeks on PGR-free MS), 28.77 and 13.50 shoots/explant were obtained, respectively. Rooting was spontaneous and regenerated plants were successfully transplanted to soil.
Gagnepainia, medicinal plant, thidiazuron, rare plant, Zingiberaceae