Journal of Plant Biochemistry and Biotechnology
  • Year: 2008
  • Volume: 17
  • Issue: 1

SSR and RAPD profile based grouping of selected jute germplasm with respect to fibre fineness trait

  • Author:
  • Javid Iqbal Mir, Pran Gobinda Karmakar, Swapan Chattopadhyay, Subrata Kumar Chaudhury, Subrata Kumar Ghosh, Anirban Roy
  • Total Page Count: 7
  • Page Number: 29 to 35

Abstract

With an aim to develop mapping population on fibre fineness trait, grouping of 16 selected jute accessions, eight each from Corchorus olitorius and Corchorus capsularis which showed promising agronomic characteristics, was carried out using fibre fineness data and DNA fingerprinting using SSR and RAPD primers. Based on fibre fineness trait two subgroups depicting the fine and coarse fibre yielding accessions were obtained in each species. A total of 26 RAPD primers and 22 pairs of SSR primers yielded 277 and 41 scorable bands, respectively. High level of polymorphism was detected between fine and coarse fibre yielding jute accessions. Dendrogram showed that all the accessions formed two main clusters between C. olitorius and C. capsularis and each main cluster subdivided in two clusters containing fine and coarse fibre jute accessions. RAPD and SSR marker data-sets showed high levels of positive correlation (Mantel test, r = 0.97). Grouping of jute accessions based on morphological and molecular data was highly correlated. This study will be useful in future jute breeding programs.

Keywords

Corchorus capsularis, Corchorus olitorius, fibre fineness, genetic diversity, parent selection, random amplified polymorphic DNA, simple sequence repeat