Plant Tissue Culture Division, Department of Biotechnology and Botany, Sri Krishnadevaraya University, Anantapur 515 003, Andhra Pradesh, India.
*Corresponding author. E-mail: pullaiaht@yahoo.co.in
Abbreviations2,4-D
2,4-Dichlorophenoxy acetic acid
2iP2-Isopentyl adenine
BAPBenzyle-6-aminopurine
MSMurshige and Skoog
KNKinetin
IAAIndole-3-acetic acid
IBAIndole-3-butyric acid
NAANaphthalene-3-acetic acid
TDZThidiazuron
Callus cultures were established from seedling explants of Pergularia daemia (Forsk) Chiov on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins. Optimal callus developed from leaf explants on MS medium supplemented with 2,4-D (2 mg l−1) + 2iP (0.1 mg l−1), was used for morphogenesis. Adventitious shoots were regenerated (70%) from the calli on MS medium supplemented with NAA (0.1 mg l−1) + BAP (2 mg l−1). Individual shoots were rooted on half strength MS medium supplemented with 0.1 mg l−1 IBA. Plantlets with well developed roots were successfully transferred to soil and 50% of the transferred plants survived.
Pergularia daemia, medicinal plant, morphogenesis, adventitious shoots, regeneration
