1Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 115, Taiwan.
2Institute of Biotechnology, National University of Kaohsiung, Kaohsiung, 811, Taiwan.
*Corresponding author: E-mail: jentsung@nuk.edu.tw
On-line published ……, 2008.
The effects of salicylic and acetylsalicylic acid on direct somatic embryogenesis were investigated using leaf explants of two cultivars of Oncidium on ½ MS medium with or without thidiazuron. In cv Gower Ramsy, salicylic acid (1, 5, 10, 15, 20, 50 μM) either alone or in combination with thidiazuron (4.54 μM) retarded and delayed embryogenesis. In contrast, in the presence of 4.54 μM of thidiazuron, acetylsalicylic acid at 0.1 μM concentration promoted embryogenesis. In cv Sweet Sugar, all concentrations of salicylic acid with or without thidiazuron proved inhibitory on embryo induction. However, in the presence of 0.45 μM thidiazuron, 0.1 and 1 μM acetylsalicylic acid promoted embryogenesis. In addition, in the presence of 4.54 μM thidiazuron, 0.01, 0.1 and 1 μM acetylsalicylic acid promoted embryogenesis.
acetylsalicylic acid, embryogenic response, leaf explant, orchid, salicylic acid, thidiazuron
