In Vitro Propagation and Conservation of Dendrobium lituiflorum Lindl through Protocorm-Like Bodies

Axillary buds obtained from 5-month-old in vitro growing plants of Dendrobium lituiflorum Lindl were cultured in Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-D. Fastest initiation (13.3 days) of protocorm-like bodies (PLBs) was observed in cultures containing MS medium supplemented with 0.5 mg l⁻¹ 2,4-D. Maximum explant response of 83% was also observed in the same medium. PLBs obtained in MS medium containing 0.5 mg l⁻¹ 2,4-D showed maximum regeneration potential of seedlings (19 explant⁻¹) when subcultured in MS medium. Well developed shoots and roots of the seedlings were obtained in the medium containing 0.5 mg l⁻¹ each of NAA and BAP, in combination. Encapsulated PLBs of D. lituiflorum could be stored at 8°C for 90 days with 80% regeneration. However, it was observed that regeneration potential of encapsulated PLBs reduced with further storage. Seventy seven per cent hardened plants survived and bloomed after 2.5 years of hardening.