1Plant Biotechnology Group, Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, Kolkata, 700 032, India.
2Plant Biotechnology Laboratory, Centre of Advanced Study, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019, India.
*Corresponding author: E-mail: mita_jm@yahoo.com
**Present Address: Head, Department of Biotechnology, Institute of Genetic Engineering, 30, Thakurhat Road, BADU, Kolkata - 700 128, India.
An attempt has been made to enhance colchicine biosynthesis in Iphigenia indica L grown in vitro by exogenous supply of precursors and elicitors during culture. Addition of tyrosine at 30 mg l−1 enhanced colchicine accumulation up to 6.1 mg g−1 dry wt (control 0.99 mg g−1 dry wt), while 25 μM NiSO4 stimulated maximum colchicine accumulation (10.25 fold of control value). The colchicine accumulation was recorded as 16.25 mg g−1 dry wt when NiSO4 was added with tyrosine.
colchicine, elicitors, Iphigenia indica, medicinal plant, micropropagation, precursors
