Journal of Plant Biochemistry and Biotechnology

  • Year: 2009
  • Volume: 18
  • Issue: 1

In Silico and Deletion Analysis of Upstream Promoter Fragment of S-Adenosyl Homocysteine Hydrolase (SAHH1) Gene of Arabidopsis Leads to the Identification of a Fragment Capable of Driving Gene Expression in Developing Seeds and Anthers

  • Author:
  • T P Sujatha, C Sivanandan, S R Bhat, R Srinivasan
  • Total Page Count: 1
  • DOI:
  • Page Number: 0 to 0

National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110 012, India.

*Corresponding author: E-mail: srinivasan53@gmail.com

Online published 3 October 2008.

Abstract

S-adenosyl homocysteine hydrolase (SAHH) is a key enzyme in methylation metabolism of eukaryotes. A 1585 bp fragment upstream to ATG of SAHH1 gene, was fused with a promoter-less β-Glucuronidase (GUS) gene and mobilized into Arabidopsis by Agrobacterium-mediated floral transformation to generate transgenic Arabidopsis. This fragment was found to drive constitutive expression of GUS in T2 progeny of transgenic Arabidopsis. In silico analysis of the promoter region of SAHH1 suggested the presence of several cis-regulatory motifs including seed-specific motifs as well as anther-specific motifs in the 376 bp (upstream to TSS of SAHH1) promoter fragment. Based on the partial deletion analysis carried out in the promoter region of SAHH1 (At4g13940, and this 376 bp promoter fragment was found to be capable of driving GUS expression in developing seeds and in some anthers/microspores.

Keywords

SAHH1, promoter, methylation, GUS, seed-specific