1Department of Biotechnology, Sri Krishnadevaraya University, Anantapur 515 003, Andhra Pradesh, India.
2Department of Botany, Sri Krishnadevaraya University, Anantapur 515 003, Andhra Pradesh, India
*Corresponding author, E-mail: Pullaiah.thammineni@gmail.com
Online published - 20 December, 2008.
A procedure for in vitro propagation of pharmaceutically valuable varieties of Caralluma adscendens from nodal explant, is described. The highest shoot multiplication with 80% frequency was achieved within one month on Murashige and Skoog's medium supplemented with 8.87μM BA. Shoot multiplication occurred in subsequent subcultures in culture bottles on MS medium. Regenerated shoots were rooted on half strength MS medium supplemented with NAA (0.54μM) in all the three varieties. The rooted plants were hardened for establishment in soil.
Caralluma adscendens, nodal explant, benzyl adenine, NAA, medicinal plant
