Journal of Plant Biochemistry and Biotechnology

  • Year: 2009
  • Volume: 18
  • Issue: 1

Housekeeping Gene Selection for Real Time-PCR Normalization in Female Hop (Humulus lupulus L) Tissues

  • Author:
  • Lina Maloukh1,2, Jaroslav Matousek3,4, Erik Van Bockstaele1,2, Isabel Roldán-Ruiz1,
  • Total Page Count: 1
  • DOI:
  • Page Number: 0 to 0

1Institute for Agricultural and Fisheries Research, Unit Plant, Growth and Development, Caritasstraat 21, 9090 Melle, Belgium.

2Ghent University, Faculty of Bioscience Engineering, Department of Plant Production, Coupure Links 653, 9000 Ghent, Belgium.

3Biology Centre of the ASCR, v.v.i. Institute of Plant Molecular Biology, Branišovská 31, 370 05 Èeské Budìjovice, Czech Republic.

4Faculty of Biological Sciences, University of South Bohemia, Branišovská 31, 37005, Èeské Budìjovice, Czech Republic.

Abstract

The variability of transcript accumulation of six genes encoding Chloropyll-a/b (Chla/b) binding protein, Nicotinamide Adenine Dinucleotide Hydride (NADH) dehydrogenase, Histone H3 (H3), DEAD-box RNA helicase 1 (DRH1) and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and of the 7SL component of the signal recognition particle (7SL-RNA), was estimated in leaves and female inflorescences of the two hop cultivars, White Golding and Admiral at different developmental stages by RT-PCR. The value of these genes as internal, normalization controls in gene transcript accumulation studies was assessed. The combination of the three housekeeping genes DRH1, GAPDH and 7SL-RNA as internal standards for hop, provided reliable results in the quantitative analysis of the transcript accumulation of Hua Enhancer 1 transcription factor (HEN1) homologue in hop tissues and is recommended for future studies of gene expression in female hop tissues.

Keywords

gene expression, Hop, Humulus lupulus, normalization, Real time-PCR