1Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110 012, India.
2Division of Plant Pathology, Indian Institute of Horticultural Research, Bangalore 560 089, India.
*Corresponding author. E-mail: rakeshjain56@yahoomail.co.in
Online published: 20 December, 2008.
Production of polyclonal antibodies requires large amount of purified virus that can be avoided by the use of recombinant coat protein (CP). Recombinant CP of Papaya ringspot virus (PRSV) was thus used for the production of polyclonal antibodies as the virus purification from papaya tissues provides low virus yields. CP was expressed as a fusion protein (∼72 kD) containing a fragment of E. coli maltose binding protein. Polyclonal antibodies from rabbits immunized with the fusion protein, successfully detected natural infection of PRSV in papaya and cucurbits samples collected from different locations at 1:4000 dilution in direct antigen-coated enzyme-linked immunosorbent assay.
PRSV, coat protein, recombinant protein, polyclonal antibodies
