1Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, 26 Hexing Road, Harbin 150040, China.
2School of Biotechnology, Kangwon National University, Chuncheon, 200–701, South Korea.
*Corresponding author: E-mail: quguanzheng@yahoo.com
Online published 12 July, 2009.
Genetic tumors can develop on certain tobacco hybrid plant spontaneously. In this study, exogenous hormones were applied on the tumorous tobacco hybrid seedlings (N. glauca × N. langsdorffii), and the data showed that N6-benzyladenine (BA) enhanced the tumor formation under both tissue culture and field conditions, and NAA and ABA inhibited tumor formation under tissue culture condition, whereas ABA accelerated the tumor formation under field condition. Furthermore, Northern analysis indicated that tumor tissue exhibited high level of CycD expression compared with normal tissues, implying abnormal cell cycle of tumor cells. The differential display reverse transcription PCR (DDRT-PCR) results indicated some genes may be involved in tumor formation.
N. glauca × N. langsdorffii, genetic tumor, hormone, CycD, DDRT-PCR
