Journal of Plant Biochemistry and Biotechnology

  • Year: 2010
  • Volume: 19
  • Issue: 1

Analysis of the Coat Protein Gene of Indian Strain of Apple stem grooving virus

  • Author:
  • Anuradha Negi, Tanuja Rana, Yogesh Kumar, Raja Ram, Vipin Hallan, Aijaz A Zaidi
  • Total Page Count: 4
  • DOI:
  • Page Number: 91 to 94

Plant Virology Lab, Institute of Himalayan Bioresource Technology (CSIR), Palampur176 061, Himachal Pradesh, India.

* Corresponding author. E-mail: zaidi_aijaz@yahoo.com

ASGV

Apple stem grooving virus;

CTLV

Citrus tatter leaf virus;

ACLSV

Apple chlorotic leaf spot virus;

ApMV

Apple mosaic virus;

CP

Coat protein.

Abstract

A survey was undertaken in the temperate fruit growing regions of Himachal Pradesh (HP) and Jammu & Kashmir (J&K). Apple stem grooving virus (ASGV), a Capillovirus, was detected in different cultivars of apple, nectarines, plum, cherry, quince and apricot by double antibody sandwich ELISA (DAS-ELISA). The coat protein (CP) gene sequence of an amplicon produced by RT-PCR, confirmed the association of ASGV in apple cultivar Starkrimson, collected from Himachal Pradesh. The CP of Indian ASGV isolate shared 100% sequence identity with a Brazilian isolate (AF438409). Sequence analysis by Recombination Detection Program (RDP2) indicated no recombination event for the Indian isolate. However, recombination was detected in Chinese, Korean and Citrus tatter leaf virus-Taiwan (CTLV) strains of ASGV. The study describes first report of ASGV infection in India and characterization of its CP gene.

Keywords

ASGV, CTLV, CP, apple, cherry, nectarine, plum, quince apple, ELISA, RT-PCR