1Plant Transformation Laboratory, National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow 226 001, India.
2Plant Physiology and Biotechnology Division, National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow 226 001, India.
* Corresponding author. E-mail: pratibhaflora@yahoo.com; pratibhamisra@nbri.res.in
AbbreviationsCAT
catalase;
EDTAethylene diaminetetra acetic acid;
NAA-αnaphthaleneacetic acid;
POXperoxidase;
PVPpolyvinylpolypyrrolidone;
PMSFphenazine methosulfate;
SODsuperoxide dismutase
A protocol for indirect differentiation of shoots/roots from leaf callus of Asiatic hybrid lily was developed through in vitro methods. The involvement of antioxidant enzymes, like, SOD, POX and CAT, and their isoenzymes during organogenesis in the morphogenetic callus was studied.The activity of these enzymes was increased during early development and differentiation of callus. SOD activity increased significantly as compared to POX and CAT during root formation, while it decreased in shoot formation and the decrease was significant in POX and CAT enzymes. The results indicate that the organogenesis is a very complicated biological process involving up and down regulation of a number of antioxidant enzymes, which seem to play an important role during organogenesis of Lilium callus.
antioxidant enzymes, Asiatic hybrid lily, isoenzymes, POX, SOD, CAT
