1Soybean Genomics and Improvement Laboratory, Plant Sciences Institute, MD 20705-3000, USA.
2Food Composition and Methods Development Laboratory, BHNRC, ARS, USDA, Beltsville, MD 20705-3000, USA.
3Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742-4452, USA.
* Corresponding author. E-mail: Savi.Natarajan@ars.usda.gov
AbbreviationsHPLC
high performance liquid chromatography;
2D-PAGEtwo-dimensional polyacrylamide gel electrophoresis;
GLMgeneral linear model.
Two soybean components namely, storage proteins and isoflavone content in a wild and three cultivated soybean genotypes were characterized and compared. The storage proteins, -conglycinin and glycinin were separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and two major storage proteins and their subunits were characterized using mass spectrometry. The three isoflavones, aglycon and the nine conjugated forms were separated by HPLC (high performance liquid chromatography) and identified by comparison of retention time, ultraviolet and mass spectral analyses. Comparison between the number of 2D-PAGE protein spots of the storage protein subunits and HPLC area of twelve isoflavones was also evaluated. The analysis of proteins and isoflavones from the wild genotype and the three cultivated genotypes suggested possible interactions between proteins and isoflavones. The same wild genotype, which showed significant statistical differences in -conglycinin and glycinin protein profiles also revealed considerable reduction in total isoflavones (> 55%) content.
proteomics, soybean, 2D-PAGE, isoflavones
