1Department of Genetics, CCS Haryana Agricultural University, Hisar, 125 004, India.
2Bioinformatics Section, CCS Haryana Agricultural University, Hisar, 125 004, India.
3Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar, 125 004, India.
4Department of Forestry, CCS Haryana Agricultural University, Hisar, 125 004, India.
*Corresponding author, E-mail: minakshisharma1@yahoo.com
Online published 12 May, 2010
A collection of male and female plants of ten Jojoba [Simmondsia chinensis (Link) Schneider] genotypes was analyzed with 50 RAPD and 55 ISSR markers to compare the efficiency and utility of these techniques for detecting genetic polymorphism. RAPD and ISSR analysis yielded 442 and 566 scorable amplified products, respectively, of which 60.7 and 69.3% were polymorphic. ISSRs revealed efficiency over RAPDs due to high EMR (effective multiplex ratio), DI (diversity index, mean PIC per primer) and MI (marker index) values. Jaccard similarity matrices among male plants, among female plants and between male and female plants of the ten jojoba genotypes varied between 0.705–0.784. Dendrograms generated by cluster analysis (UPGMA, NTSYS-pc) supported by bootstrap values using RAPD and ISSR datasets led to grouping of most of male and females genotypes in separate clusters. While pattern of clustering remained more or less same, the two dendrograms did differ with respect to the grouping of a few male and female genotypes. The value of the Mantel test shows poor correlation (r = 0.41) between ISSR and RAPD marker datasets.
genetic diversity, ISSR, jojoba, molecular markers, RAPD
