Bioctechnology Centre, Indian Agricultural Research Institute, New Delhi 110 012, India
* Corresponding author.
Vicilin, one of the major storage proteins of chickpea (Cicer arietinum L.) was purified and characterized during seed development. Vicilin was purified by zonal isoelectric precipitation followed by chromatography on DEAE-cellulose column. Vicilin on SDS-PAGE resolved into 5 major bands ranging in mol wt from 1.4 to 66 kD. More heterogenous pattern emerged on isoelectric focussing. This protein had high amount of amides and low amount of sulphur containing amino acids.
chickpea, storage proteins, vicilin, isoelectric focussing
